Taking into consideration the lead of cardiovascular diseases among worldwide morbidity and mortality, raising prevalence of concomitant cases of type 2 diabetes mellitus (DM2) and chronic heart failure (CHF) demands particular attention. Molecular landscape of CHF corresponds to severe mitochondrial dysfunction boosted by diabetes associated metabolic abnormalities. Therefore our research is concentrated on establishing its predictive role in early CHF development in patients with DM2. We performed a live cell assay of mitochondrial respiration rate in vivo for patients with CHF and diabetes mellitus and established the degree of their mitochondrial dysfunction in comparison to the control group of relatively healthy individuals. The research comprised several stages including venous blood sampling and isolation of preripheral blood mononuclear cells (PBMC)  followed by the mitochondrial respirartion rate assessments using Seahorse XFe96 Extracellular Flux Analyzer. Statistical analysis was performed suing QI Macros statistical software package. Regarding groups with CHF, mitochondrial respiration of comorbid patients with diabetes is suppressed to a much greater extent. Moreover, presence of concomitant to CHF diabetes mellitus abolishes the nonmitochondrial respiration to a limited degree. 

Our results not only confirm the paradigmatic nature of DM2 as an independent indicator for CHF but also reveal its predisposition to CHF development at a molecular level even in relatively healthy individuals.

Polina Oktysyuk has graduated from S.M. Kirov Military Medical Academy at the age of 24. She is currently a resident doctor in the Internal Diseases Propadeutics Department clinic of the Academy, Saint Petersburg Russian Federation.